In vitro synthesis of biologically active human leukocyte interferon in a RNA-dependent system from Saccharomyces cerevisiae

Proc Natl Acad Sci U S A. 1983 May;80(9):2569-73. doi: 10.1073/pnas.80.9.2569.

Abstract

RNA isolated from yeast Saccharomyces cerevisiae containing recombinant plasmid DNA coding for a human leukocyte interferon gene was used as template in a modified yeast cell-free translation system. Radioactive as well as biologically active leukocyte interferon was synthesized.

MeSH terms

  • Cell-Free System
  • Humans
  • Interferon Type I / biosynthesis*
  • Interferon Type I / genetics
  • Plasmids
  • Protein Biosynthesis
  • RNA / metabolism*
  • Saccharomyces cerevisiae / metabolism*
  • Templates, Genetic

Substances

  • Interferon Type I
  • RNA