A monoclonal antibody against human colonic adenoma recognizes difucosylated Type-2-blood-group chains

Biosci Rep. 1983 Feb;3(2):163-70. doi: 10.1007/BF01121947.

Abstract

A monoclonal antibody C14/1/46/10 showing preferential binding to membranes of human colorectal carcinomas over normal colon mucosae was obtained by immunization of mice with extra-nuclear membranes of a human colonic adenoma. Binding and inhibition of binding assays using blood cells or glycoproteins with known blood-group activities indicated that the antibody recognizes a carbohydrate antigen co-existing with the blood-group-H determinant: Fuc alpha 1 leads to 2 Gal. Inhibition assays with structurally defined oligosaccharides showed that the antigenic determinant involves difucosylated Type-2-blood-group chains with the structure: (formula; see text)

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoma / immunology*
  • Animals
  • Antibodies, Monoclonal / isolation & purification*
  • Antibodies, Neoplasm*
  • Binding Sites, Antibody
  • Blood Group Antigens / immunology*
  • Colonic Neoplasms / immunology*
  • Dogs
  • Epitopes
  • Humans
  • Intestinal Mucosa / immunology
  • Mice
  • Mice, Inbred BALB C
  • Swine

Substances

  • Antibodies, Monoclonal
  • Antibodies, Neoplasm
  • Blood Group Antigens
  • Epitopes