Meiotic Resumption and Intracellular cAMP Levels in Mouse Oocytes Treated With Compounds Which Act on cAMP Metabolism

Cell Differ. 1983 May;12(5):271-6. doi: 10.1016/0045-6039(83)90023-4.


We have studied the effect of agents known to stimulate adenylate cyclase on spontaneous meiotic resumption in vitro by mouse oocytes. We have found that cholera toxin (CT) (up to 1 microM) and prostaglandin E1 (PGE1) (up to 160 microM) are not able to prevent meiotic resumption, but a clear dose-dependent delay in meiosis resumption was observed during the first 3 h of incubation in medium containing CT or PGE1. The effect became clearer when a small concentration of isobutylmethylxanthine (MIX) (1 microM) was added to the medium. We have also measured the cAMP content of the oocyte: the basal content is 2.1 fmol; this value drops to 0.9 fmol during a 2-h culture period. The decrease is partially prevented if CT is present in the incubation medium, while total cAMP content increases to 3.1 fmol in the presence of 1 mM MIX. The results suggest that isolated mouse oocytes contain toxin and prostaglandin sensitive adenylate cyclase and also an active phosphodiesterase system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-Methyl-3-isobutylxanthine / pharmacology
  • Adenylyl Cyclases / metabolism
  • Alprostadil
  • Animals
  • Cholera Toxin / pharmacology
  • Cyclic AMP / metabolism*
  • Female
  • Meiosis / drug effects*
  • Mice
  • Oocytes / drug effects
  • Oocytes / metabolism*
  • Ovum / metabolism*
  • Prostaglandins E / pharmacology


  • Prostaglandins E
  • Cholera Toxin
  • Cyclic AMP
  • Adenylyl Cyclases
  • Alprostadil
  • 1-Methyl-3-isobutylxanthine