Detection of Candida proteinase by enzyme immunoassay and interaction of the enzyme with alpha-2-macroglobulin

J Immunol Methods. 1983 Jun 24;61(1):107-16. doi: 10.1016/0022-1759(83)90014-5.


Double antibody immunoassay of secretory aspartic proteinases from 2 strains of the yeast Candida albicans was performed in microtest plates with rabbit antibody and peroxidase conjugated rabbit antibody. The test detects approximately 0.1 ng of proteinase and is 2 orders of magnitude more sensitive than direct measurement of enzymic activity with hemoglobin as a substrate. The immunoassay was affected both by alkaline denaturation, which is a common feature of aspartic proteinases, and by the presence of the proteinase scavenger alpha-2-macroglobulin. Conditions are described that allow for comparison of proteinases from different strains of yeast and minimize the interference of macroglobulin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Candida albicans / enzymology*
  • Cattle
  • Electrophoresis, Polyacrylamide Gel
  • Hydrogen-Ion Concentration
  • Immune Sera
  • Immunoelectrophoresis
  • Immunoenzyme Techniques
  • Peptide Hydrolases / analysis*
  • Peptide Hydrolases / immunology
  • alpha-Macroglobulins*


  • Immune Sera
  • alpha-Macroglobulins
  • Peptide Hydrolases