Primary structure of the oligosaccharide determinant of blood group Cad specificity

J Biol Chem. 1983 Jun 25;258(12):7691-5.


Glycophorin A and B from Cad erythrocyte membranes are the carriers of the blood group Cad determinants. They are characterized by a significant increase in molecular mass, as compared to the corresponding glycophorins from control erythrocytes (Cartron, J.-P., and Blanchard, D. (1982) Biochem. J. 207, 497-504). Lipid-free glycophorin A, purified from Cad red cells, showed an increased GalNAc content in comparison to blood group B, Cad-negative, control cells. Alkaline-borohydride treatment of this Cad glycophorin A released as a predominant species a pentasaccharide; its structure was determined, by methylation analysis and by 500-MHz 1H-NMR studies, to be: (formula; see text) This novel oligosaccharide inhibited strongly the hemagglutination of Cad erythrocytes by the Dolichos biflorus lectin. It shares with the blood group Sda determinant a terminal GalNAc beta (1 leads to 4)Gal beta (1 leads to .) sequence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Group Antigens / genetics*
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Epitopes / analysis*
  • Erythrocyte Membrane / immunology*
  • Erythrocytes / immunology*
  • Glycophorins / immunology*
  • Humans
  • Magnetic Resonance Spectroscopy
  • Oligosaccharides / immunology*
  • Sialoglycoproteins / immunology*


  • Blood Group Antigens
  • Epitopes
  • Glycophorins
  • Oligosaccharides
  • Sialoglycoproteins