Site of tubulin synthesis in Tetrahymena pyriformis

FEBS Lett. 1983 Jul 25;158(2):271-5. doi: 10.1016/0014-5793(83)80593-6.


Free and membrane-bound polysomes were isolated from the protozoa Tetrahymena pyriformis, and the contribution of these two types of polysomes to tubulin synthesis were studied using immunoprecipitation of the 35S-translational products in a rabbit reticulocyte lysate. One-dimensional electrophoretic analysis shows that tubulin is synthesized by polyadenylated RNA isolated from free and membrane-bound polysomes. Non-polyadenylated RNAs of free polysomes are also able to direct tubulin synthesis. Two-dimensional electrophoretic analysis using O'Farrell's system confirms these results and also reveals the existence of the alpha- and beta-tubulin subunits.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites
  • Immune Sera / analysis
  • In Vitro Techniques
  • Poly A / metabolism
  • Polyribosomes / metabolism
  • Protein Biosynthesis
  • RNA / metabolism
  • RNA, Messenger
  • Rabbits
  • Tetrahymena pyriformis / metabolism*
  • Tubulin / biosynthesis*


  • Immune Sera
  • RNA, Messenger
  • Tubulin
  • Poly A
  • RNA