T-cell hybridoma specific for a cytochrome c peptide: specific antigen binding and interleukin 2 production

Proc Natl Acad Sci U S A. 1983 Aug;80(15):4832-6. doi: 10.1073/pnas.80.15.4832.


T-cell hybridomas were obtained after fusion of BW 5147 thymoma and long-term cultured T cells specific for cytochrome c peptide 66-80 derivatized with a 2,4-dinitroaminophenyl (DNAP) group. The resulting hybridomas were selected for their capacity to specifically bind to soluble radiolabeled peptide antigen. One T-cell hybrid was positive for antigen binding. This hybrid T cell exhibits surface phenotypic markers of the parent antigen-specific T cells. The binding could be inhibited either by an excess of unlabeled homologous antigen or by cytochrome c peptide 11-25 derivatized with a 2-nitrophenylsulfenyl group. Several other peptide antigens tested failed to inhibit binding of the radioactive peptide. This suggests that a specific amino acid sequence, modified by a DNAP group, is the antigenic structure recognized by the putative T-cell receptor. In addition, direct interaction of DNAP-66-80 peptide with the hybridoma cell line induced production of the T-cell growth factor interleukin 2. Furthermore, supernatants derived from syngeneic macrophages pulsed with the relevant peptide also induced the antigen-specific hybridoma to produce interleukin 2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cells, Cultured
  • Cytochrome c Group / immunology*
  • Epitopes / analysis*
  • Female
  • Hybridomas / immunology*
  • Interleukin-2 / biosynthesis*
  • Macrophages / immunology
  • Mice
  • Mice, Inbred AKR
  • Mice, Inbred Strains
  • Neoplasms, Experimental / immunology
  • Peptide Fragments / immunology
  • T-Lymphocytes / immunology*
  • Thymoma / immunology
  • Thymus Neoplasms / immunology


  • Cytochrome c Group
  • Epitopes
  • Interleukin-2
  • Peptide Fragments