Selective increase in cytokeratin synthesis in cultured rat hepatocytes in response to hormonal stimulation

Biochem Biophys Res Commun. 1983 Jul 29;114(2):556-63. doi: 10.1016/0006-291x(83)90816-1.


Addition of a combination of insulin, dexamethasone and EGF at seeding time to cultured rat hepatocytes in serum-free medium caused a selective increase in the biosynthesis of particular cytokeratin components. This increase was prominent during the first day in culture. No significant increases were detected in the absence of hormones or in the presence of either hormones added alone or in pairs, except in the case of insulin plus dexamethasone, which yielded an effect close to that obtained with the three factors. Interestingly, the latter condition also maintained a high level of albumin production over a 6-day period in culture.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging
  • Animals
  • Dexamethasone / pharmacology*
  • Epidermal Growth Factor / pharmacology*
  • In Vitro Techniques
  • Insulin / pharmacology*
  • Keratins / biosynthesis*
  • Liver / drug effects
  • Liver / growth & development
  • Liver / metabolism*
  • Methionine / metabolism
  • Molecular Weight
  • Proteins / genetics
  • Proteins / isolation & purification
  • Rats
  • Rats, Inbred F344


  • Insulin
  • Proteins
  • Epidermal Growth Factor
  • Keratins
  • Dexamethasone
  • Methionine