Hybridization of nucleic acids directly in agarose gels

Anal Biochem. 1983 Jun;131(2):365-72. doi: 10.1016/0003-2697(83)90185-9.

Abstract

Nucleic acids, both DNA and RNA, separated on agarose gels can be visualized by direct hybridization of the dried gel with appropriate radioactive probes. This method does not involve the transfer of the nucleic acid from the gel. The method requires less manipulation than other procedures; it is extremely rapid, sensitive, and inexpensive. These attributes make this procedure a valuable alternative or supplement to the commonly used methods for visualization by hybridization of nucleic acids separated on agarose gels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophages / analysis
  • DNA / isolation & purification*
  • Electrophoresis, Agar Gel / methods
  • Molecular Weight
  • Nucleic Acid Hybridization*
  • Plasmids
  • RNA / isolation & purification*

Substances

  • RNA
  • DNA