Localization of antigenic determinants in whole Drosophila embryos

Dev Biol. 1983 Sep;99(1):261-4. doi: 10.1016/0012-1606(83)90275-0.

Abstract

Procedures are described for removal of the vitelline membrane from gently fixed Drosophila embryos en masse. The resulting embryos retain excellent structural integrity and are now suitable for a variety of immunocytochemical and biochemical characterizations.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Drosophila melanogaster / embryology*
  • Embryo, Nonmammalian / immunology
  • Epitopes
  • Fixatives
  • Fluorescent Antibody Technique

Substances

  • Antibodies, Monoclonal
  • Epitopes
  • Fixatives