Morphological, cytochemical and ultrastructural studies of a case of systemic mastocytosis

Basic Appl Histochem. 1983;27(3):183-90.


Morphological, cytochemical and ultrastructural studies of mast cells were carried out in a patient affected with systemic mastocytosis. Neoplastic mast cells showed morphological features between classic tissue mast cells and circulating basophils. They showed strong granule metachromasia after toluidine blue, faint positivity to Hotchkiss reaction, strong positivity to chloroacetate esterase, while they were negative to alkaline phosphatase. Ultrastructural observations showed heterogeneity of granules, most of which had homogenous fine dotted contents. The origin and linkage between mast cells and circulating basophils are discussed.

Publication types

  • Case Reports

MeSH terms

  • Alkaline Phosphatase
  • Bone Marrow / pathology
  • Carboxylic Ester Hydrolases
  • Humans
  • Liver / pathology
  • Lymph Nodes / pathology
  • Male
  • Mast Cells / pathology*
  • Mast Cells / ultrastructure
  • Microscopy, Electron
  • Middle Aged
  • Periodic Acid-Schiff Reaction
  • Staining and Labeling / methods
  • Tolonium Chloride
  • Urticaria Pigmentosa / pathology*


  • Tolonium Chloride
  • Carboxylic Ester Hydrolases
  • chloroacetate esterase
  • Alkaline Phosphatase