Prevention of defects of axonal transport and nerve conduction velocity by oral administration of myo-inositol or an aldose reductase inhibitor in streptozotocin-diabetic rats

Diabetologia. 1983 Nov;25(5):433-8. doi: 10.1007/BF00282524.

Abstract

The effects of orally-administered myo-inositol have been compared with those of an aldose reductase inhibitor on acute neurological defects in experimentally diabetic rats. Three groups of streptozotocin-treated diabetic rats (50 mg/kg, IP) together with three groups of age-matched controls (saline, IP) were compared. One pair of groups (control and diabetic) were untreated for 3 weeks, another pair of groups received daily oral myo-inositol (667 mg/kg) and the third pair received an aldose reductase inhibitor (ICI 105 552; 50 mg . kg-1, day-1, orally). The untreated diabetic group showed statistically significant deficits in accumulation, proximal to 24 h sciatic nerve constrictions, of choline acetyltransferase activity by comparison with untreated controls (2.8 +/- 0.4 versus 5.1 +/- 0.4 nmol acetylcholine . h-1 . nerve-1; p less than 0.001). The untreated diabetic rats also showed a fall in motor nerve conduction velocity of 6.2 +/- 0.7 m/s which was statistically significant (p less than 0.001). Treatment of the diabetic group with myo-inositol prevented the development of both defects of axonal transport and conduction velocity; both measurements were similar to those of the myo-inositol treated control rats. Likewise the diabetic rats which received aldose reductase inhibitor showed prevention of both defects. Nerves from untreated diabetic rats showed marked sorbitol accumulation and a statistically significant reduction in myo-inositol content by comparison with the untreated controls (sorbitol, 1.56 +/- 0.22 versus 0.8 +/- 0.01 and myo-inositol, 1.47 +/- 0.10 versus 2.3 +/- 0.10 nmol/mg; p less than 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aldehyde Reductase / antagonists & inhibitors*
  • Animals
  • Axonal Transport / drug effects*
  • Blood Glucose / analysis
  • Diabetes Mellitus, Experimental / physiopathology*
  • Diabetic Neuropathies / physiopathology*
  • Diabetic Neuropathies / prevention & control
  • Inositol / metabolism
  • Inositol / therapeutic use*
  • Male
  • Motor Neurons / drug effects
  • Motor Neurons / physiology
  • Neural Conduction / drug effects*
  • Quinolines / therapeutic use*
  • Quinolones*
  • Rats
  • Rats, Inbred Strains
  • Sciatic Nerve / drug effects
  • Sciatic Nerve / physiopathology*
  • Sorbitol / metabolism
  • Streptozocin
  • Sugar Alcohol Dehydrogenases / antagonists & inhibitors*

Substances

  • Blood Glucose
  • Quinolines
  • Quinolones
  • Inositol
  • Sorbitol
  • Streptozocin
  • ICI 105552
  • Sugar Alcohol Dehydrogenases
  • Aldehyde Reductase