Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Jan;114(1):121-30.

Immunohistochemical Distinction of Human Carcinomas by Cytokeratin Typing With Monoclonal Antibodies

Free PMC article

Immunohistochemical Distinction of Human Carcinomas by Cytokeratin Typing With Monoclonal Antibodies

E Debus et al. Am J Pathol. .
Free PMC article

Abstract

Carcinomas of different origin have been tested in immunofluorescence microscopy with the monoclonal murine antibodies CK1-CK4, which recognize a single cytokeratin polypeptide (human cytokeratin No. 18) present in simple but not in stratified squamous epithelia, and with the monoclonal antibody KG8.13 and guinea pig kerA antibodies, both of which recognize a variety of cytokeratins common to almost all epithelial cell types. Tumors derived from simple epithelia, including adenocarcinomas and some other tumors such as ductal breast carcinomas, were strongly stained by all three antibodies. So was a transitional carcinoma of the bladder. In contrast, basal cell epithelioma, cloacogenic carcinoma, and squamous cell carcinoma of skin, tongue, and esophagus appeared negative with CK1-CK4 but positive with the other two antibodies. Other squamous cell carcinomas derived from epiglottis and cervix uteri showed a mixture of positive and negative cells when tested with CK1-CK4, although all tumor cells were positive when tested with KG8.13 and with kerA. Thus, use of an appropriate collection of cytokeratin antibodies with different specificities not only allows tumors of epithelial origin to be distinguished from other tumor types but, in addition, allows a further subdivision of carcinomas in relation to their histologic origin.

Similar articles

See all similar articles

Cited by 41 articles

See all "Cited by" articles

References

    1. Nature. 1975 Aug 7;256(5517):495-7 - PubMed
    1. J Clin Pathol. 1982 Nov;35(11):1253-67 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2813-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 - PubMed
    1. Cell. 1980 Nov;22(1 Pt 1):17-25 - PubMed

Publication types

LinkOut - more resources

Feedback