Structure of the O-chain of the Phenol-Phase Soluble Cellular Lipopolysaccharide of Yersinia Enterocolitica Serotype O:9

Eur J Biochem. 1984 Feb 15;139(1):195-200. doi: 10.1111/j.1432-1033.1984.tb07994.x.

Abstract

The phenol-phase soluble cellular lipopolysaccharide isolated by the phenol/water extraction method from Yersinia enterocolitica serotype O:9 cells was shown by hydrolytic, periodate oxidation, methylation and nuclear magnetic resonance studies to be an S-type lipopolysaccharide with a linear O-antigenic polysaccharide of 1,2-linked 4,6-dideoxy-4-formamido-alpha-D-mannopyranosyl units. The serological cross-reactivity between Y. enterocolitica serotype O:9 and the lipopolysaccharides of Vibrio cholerae and Brucella species can now be related to the presence of N-acylated 4-amino-4,6-dideoxy-alpha-D-mannopyranosyl residues in their respective O-antigenic chains.

MeSH terms

  • Antigens, Bacterial / isolation & purification*
  • Chemical Phenomena
  • Chemistry
  • Immunochemistry
  • Lipid A
  • Lipopolysaccharides / isolation & purification*
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • O Antigens
  • Phenol
  • Phenols
  • Serotyping
  • Solubility
  • Yersinia / immunology*

Substances

  • Antigens, Bacterial
  • Lipid A
  • Lipopolysaccharides
  • O Antigens
  • Phenols
  • Phenol