Characterization of virus-like particles from a psoriatic patient with respect to the possible presence of particle-associated RNA and RNA-directed DNA polymerase

Acta Pathol Microbiol Immunol Scand B. 1983 Dec;91(6):413-7. doi: 10.1111/j.1699-0463.1983.tb00069.x.

Abstract

Subcellular particles resembling retroviruses with respect to their morphology, density, and protein composition, and which are present in the urine of a psoriatic patient have been analysed for the presence of high-molecular-weight polyadenylated RNA and RNA-directed DNA polymerase activity. Neither RNA-directed DNA polymerase activity nor high-molecular-weight polyadenylated RNA was detected in preparations containing the purified virus-like particles. This was the case even when the amount of particles analysed exceeded by a factor of 50 or more the amount of avian myeloblastosis virus in which both polymerase activity and RNA were detected.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Humans
  • Molecular Weight
  • Poly A / analysis*
  • Psoriasis / microbiology*
  • RNA / analysis*
  • RNA, Messenger
  • RNA, Viral / analysis*
  • RNA-Directed DNA Polymerase / metabolism*
  • Virion / analysis*
  • Virion / enzymology
  • Virion / isolation & purification

Substances

  • RNA, Messenger
  • RNA, Viral
  • Poly A
  • RNA
  • RNA-Directed DNA Polymerase