Nucleotide sequence of nuclear 5.4 S RNA of mouse cells

Biochim Biophys Acta. 1984 Jun 16;782(2):127-31. doi: 10.1016/0167-4781(84)90015-0.

Abstract

The nucleotide sequence of nuclear 5.4 S RNA, a new species of small nuclear RNA (snRNA) of mouse cells, was determined. The 5.4 S RNA consists of 138 nucleotide residues containing 1 mol each of 2,2,7- trimethylguanosine (m3(2,2,7) G), 2'-O-methyladenosine (Am), 2'-O-methyluridine (Um) and pseudouridine as modified nucleosides. This RNA has a cap structure, m3(2,2,7) ++GpppAm -, at its 5'-terminus and sequences complementary to the terminal consensus sequences of introns. The sequence complementary to the 5'-splice junction, A-U-C-C-psi-U-A-C-C-U-G, is very similar to the 5'-terminal sequence of U1 RNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line
  • Leukemia, Experimental
  • Mice
  • Nucleic Acid Conformation
  • Purines / analysis
  • Pyrimidines / analysis
  • RNA / genetics*
  • RNA / isolation & purification
  • RNA Caps / isolation & purification
  • RNA Splicing
  • RNA, Small Nuclear
  • Ribonuclease T1
  • Ribonuclease, Pancreatic

Substances

  • Purines
  • Pyrimidines
  • RNA Caps
  • RNA, Small Nuclear
  • RNA
  • Ribonuclease T1
  • Ribonuclease, Pancreatic

Associated data

  • GENBANK/M14414