Anti-acetylcholine receptor response achieved by immunization with a synthetic peptide from the receptor sequence

Biochem Biophys Res Commun. 1984 Jun 15;121(2):673-9. doi: 10.1016/0006-291x(84)90234-1.

Abstract

A synthetic peptide corresponding to the first twenty amino acids of the N-terminal region from the alpha-subunit of the Torpedo acetylcholine receptor cross reacts with antibodies to the receptor. A conjugate of this peptide to bovine serum albumin elicits in rabbits an immune response towards the synthetic peptide as well as towards the acetylcholine receptor. Blotting experiments demonstrate that the antipeptide antibodies react exclusively with the alpha-subunit of the acetylcholine receptor. Antibodies against synthetic peptides from various regions of the receptor sequence may provide useful reagents for structural and developmental analysis of the acetylcholine receptor as well as for the regulation of experimental autoimmune myasthenia gravis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibody Formation
  • Epitopes
  • Immunization
  • Peptide Fragments / chemical synthesis
  • Peptide Fragments / immunology*
  • Peptide Fragments / metabolism
  • Rabbits
  • Receptors, Cholinergic / immunology*
  • Receptors, Cholinergic / metabolism
  • Receptors, Nicotinic / chemical synthesis
  • Receptors, Nicotinic / immunology*
  • Receptors, Nicotinic / metabolism
  • Torpedo

Substances

  • Epitopes
  • Peptide Fragments
  • Receptors, Cholinergic
  • Receptors, Nicotinic
  • Torpedo acetylcholine receptor, alpha-subunit 1-20