Mechanism of control of DNA replication and incompatibility in ColE1-type plasmids--a review

Gene. 1984 Apr;28(1):1-15. doi: 10.1016/0378-1119(84)90082-9.


The related phenomena of ColE1 DNA replication control and plasmid incompatibility are analysed in detail. Replication is negatively regulated in different ways by two gene products, RNA I and gene rop-coded polypeptide, while plasmid incompatibility is specified only by RNA I. Thus, copy-number mutants in the RNA I region usually have altered incompatibility and vice versa. RNA I is transcribed from the opposite strand to RNA II; the processed RNA II transcript serves as the primer for DNA replication. The base-pairing interaction between RNA I and RNA II controls DNA synthesis and incompatibility by preventing primer formation. A model is proposed to explain the phenotype of RNA I mutants in terms of the changes they cause in the secondary structure of RNA I or RNA II.

Publication types

  • Review

MeSH terms

  • Bacteriocin Plasmids*
  • DNA Replication*
  • DNA, Bacterial / biosynthesis
  • DNA, Bacterial / genetics
  • Gene Expression Regulation
  • Genes, Bacterial*
  • Models, Genetic
  • Nucleic Acid Conformation
  • Plasmids*
  • RNA / genetics*
  • RNA, Bacterial / genetics*


  • DNA, Bacterial
  • RNA primers
  • RNA, Bacterial
  • RNA