Cellular composition of a cerebral hemisphere primary culture

Abstract

In this overview attention is given to available markers and methods for characterizing cell elements in a culture system. Primary cultures from newborn rat cerebral hemispheres were grown for 14 days. The population of cells was dominated by astrocytic glial cells (60-70%), but cells with properties of macrophages, endothelial-like cells, mesenchymal-like cells, ependymal-like cells, and oligoblasts were also found. Neither mature neurons nor oligodendroglial cells were observed. The enrichment in astroglial-like cells makes the cultures a satisfactory astroglial-cell model, at least for some purposes.