A rapid method for the inactivation of virus infectivity prior to assay for interferons

J Virol Methods. 1984 Jul;8(4):349-51. doi: 10.1016/0166-0934(84)90072-7.

Abstract

Virus infectivity in samples of culture medium or suspensions of animal tissue which are required for interferon assay can be rapidly and conveniently inactivated by overnight incubation with beta-propiolactone (BPL). As BPL hydrolyses spontaneously samples can be assayed with no further treatment. BPL does not affect the interfering activity of alpha, beta or gamma mouse interferons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / microbiology
  • Cells, Cultured
  • Interferons / analysis*
  • Lactones / pharmacology*
  • Mice
  • Propiolactone / pharmacology*
  • Semliki forest virus / drug effects
  • Semliki forest virus / pathogenicity
  • Virulence / drug effects
  • Viruses / drug effects*
  • Viruses / pathogenicity

Substances

  • Lactones
  • Propiolactone
  • Interferons