Abstract
Virus infectivity in samples of culture medium or suspensions of animal tissue which are required for interferon assay can be rapidly and conveniently inactivated by overnight incubation with beta-propiolactone (BPL). As BPL hydrolyses spontaneously samples can be assayed with no further treatment. BPL does not affect the interfering activity of alpha, beta or gamma mouse interferons.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Brain / microbiology
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Cells, Cultured
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Interferons / analysis*
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Lactones / pharmacology*
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Mice
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Propiolactone / pharmacology*
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Semliki forest virus / drug effects
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Semliki forest virus / pathogenicity
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Virulence / drug effects
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Viruses / drug effects*
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Viruses / pathogenicity
Substances
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Lactones
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Propiolactone
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Interferons