Abstract
We describe an improved high-performance liquid chromatographic method for the determination of tyrosine, 5-hydroxytryptamine, 5-hydroxyindoleacetic acid, tryptophan and homovanillic acid in cerebrospinal fluid and nerve tissue, using the new microbore cartridges with 5 micron average particle size. The first four substances are quantified fluorometrically and the last two electrochemically. Both detectors are connected to the same integrator through a relay which can be switched as required. Data are collected in an on-line personal computer and evaluated statistically. An improvement in the method for extraction and separation of catecholamines is also reported.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Brain Chemistry
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Catecholamines / analysis
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Catecholamines / cerebrospinal fluid
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Chromatography, High Pressure Liquid / methods
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Electrochemistry
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Homovanillic Acid / analysis
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Homovanillic Acid / cerebrospinal fluid
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Humans
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Hydroxyindoleacetic Acid / analysis
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Hydroxyindoleacetic Acid / cerebrospinal fluid
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Microcomputers
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Nerve Tissue / analysis*
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Neurotransmitter Agents / analysis
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Neurotransmitter Agents / cerebrospinal fluid*
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Rats
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Serotonin / analysis
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Serotonin / cerebrospinal fluid
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Spectrometry, Fluorescence
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Spinal Cord / analysis
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Tryptophan / analysis
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Tryptophan / cerebrospinal fluid
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Tyrosine / analysis
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Tyrosine / cerebrospinal fluid
Substances
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Catecholamines
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Neurotransmitter Agents
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Serotonin
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Tyrosine
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Hydroxyindoleacetic Acid
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Tryptophan
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Homovanillic Acid