Characterization of substance P(1-7) and (1-8) generating enzyme in human cerebrospinal fluid

Biochem Biophys Res Commun. 1984 Nov 30;125(1):244-50. doi: 10.1016/s0006-291x(84)80360-5.


A substance P-hydrolyzing endopeptidase has been purified from a large quantity of human cerebrospinal fluid by ion exchange chromatography (DEAE-Sepharose CL-6B) and molecular sieving (Sephadex G-100 and Sephacryl S-200). The purification was monitored by measuring the conversion of synthetic substance P using a radioimmunoassay specific for its (1-7) fragment. The enzyme has an apparent molecular weight of 43,000. It cleaves predominantly at the Phe7-Phe8 and Phe8-Gly9 bonds but gives no or negligible conversion of the other tachykinins, neuromedin K and L (substance K).

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • Endopeptidases / cerebrospinal fluid*
  • Humans
  • Kinetics
  • Molecular Weight
  • Substance P / cerebrospinal fluid*
  • Substrate Specificity


  • Substance P
  • Endopeptidases