Characterization of a factor inducing differentiation of mouse myeloid leukemic cells purified from conditioned medium of mouse Ehrlich ascites tumor cells

FEBS Lett. 1984 Dec 10;178(2):291-6. doi: 10.1016/0014-5793(84)80619-5.

Abstract

A factor inducing differentiation of mouse myeloid leukemic cells (MI) into macrophages was purified to apparent homogeneity from 168 1 of CM of Ehrlich ascites tumor cells. The purified factor was half-maximally active at 2 X 10(-11) M. The factor was analyzed by radioiodination, SDS-polyacrylamide gel electrophoresis and autoradiography. Its Mr was 40 000-50 000. On reduction, the factor lost activity, but showed no subunit structure. Treatment of the factor with endo-beta-N-acetylglucosaminidase F, but not endo-beta-N-acetylglucosaminidase H, gave rise to a molecule of Mr 20 000-28 000. The activity of the factor from Ehrlich cells was completely neutralized by antiserum to the factor of Mr 50 000-70 000 from mouse fibroblast L929 cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carcinoma, Ehrlich Tumor / metabolism*
  • Cell Differentiation / drug effects
  • Epitopes / immunology
  • Glycoproteins / immunology
  • Glycoproteins / isolation & purification
  • Glycoproteins / pharmacology*
  • Glycoside Hydrolases / pharmacology
  • Growth Inhibitors*
  • Immune Sera / immunology
  • Interleukin-6*
  • L Cells / analysis
  • Leukemia Inhibitory Factor
  • Leukemia, Myeloid / pathology*
  • Lymphokines*
  • Macrophages / pathology
  • Mice
  • Mice, Inbred ICR
  • Molecular Weight

Substances

  • Epitopes
  • Glycoproteins
  • Growth Inhibitors
  • Immune Sera
  • Interleukin-6
  • Leukemia Inhibitory Factor
  • Lif protein, mouse
  • Lymphokines
  • Glycoside Hydrolases