Identification and quantification of dehydroepiandrosterone sulphate in saliva

Steroids. 1982 Jan;39(1):63-71. doi: 10.1016/0039-128x(82)90126-x.


3 beta-Hydroxy-5-androsten-17-one (dehydroepiandrosterone) sulphate has been separated from an extract of human saliva by ion exchange gel chromatography and identified by high resolution gas chromatography-high resolution mass spectrometry of the tert-butyldimethylsilyl derivative of the neutral steroid obtained by enzymic hydrolysis. Quantitative analyses, employing 7,7-2H-dehydroepiandrosterone sulphate as the internal standard, have indicated concentrations in the saliva of young adult subjects to be generally in the range 0.9-5.7 nmol/l, though concentrations as high as 10.7 nmol/l have been observed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Chromatography, Ion Exchange
  • Dehydroepiandrosterone / analogs & derivatives*
  • Dehydroepiandrosterone / analysis
  • Dehydroepiandrosterone Sulfate
  • Female
  • Gas Chromatography-Mass Spectrometry
  • Humans
  • Male
  • Saliva / analysis*


  • Dehydroepiandrosterone
  • Dehydroepiandrosterone Sulfate