Role of fructose 2,6-bisphosphate in the stimulation of glycolysis by anoxia in isolated hepatocytes

Biochem J. 1982 Aug 15;206(2):359-65. doi: 10.1042/bj2060359.

Abstract

1. Incubation of hepatocytes from fed or starved rats with increasing glucose concentrations caused a stimulation of lactate production, which was further increased under anaerobic conditions. 2. When glycolysis was stimulated by anoxia, [fructose 2,6-bis-phosphate] was decreased, indicating that this ester could not be responsible for the onset of anaerobic glycolysis. In addition, the effect of glucose in increasing [fructose 2,6-bisphosphate] under aerobic conditions was greatly impaired in anoxic hepatocytes. [Fructose 2,6-bisphosphate] was also diminished in ischaemic liver, skeletal muscle and heart. 3. The following changes in metabolite concentration were observed in anaerobic hepatocytes: AMP, ADP, lactate and L-glycerol 3-phosphate were increased; ATP, citrate and pyruvate were decreased: phosphoenolpyruvate and hexose 6-phosphates were little affected. Concentrations of adenine nucleotides were, however, little changed by anoxia when hepatocytes from fed rats were incubated with 50 mM-glucose. 4. The activity of ATP:fructose 6-phosphate 2-phosphotransferase was not affected by anoxia but decreased by cyclic AMP. 5. The role of fructose 2,6-bisphosphate in the regulation of glycolysis is discussed.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Anaerobiosis
  • Animals
  • Fructosediphosphates / metabolism*
  • Glucose / pharmacology
  • Glycolysis* / drug effects
  • Hexosediphosphates / metabolism*
  • In Vitro Techniques
  • Lactates / metabolism
  • Lactic Acid
  • Liver / cytology
  • Liver / drug effects
  • Liver / metabolism*
  • Phosphofructokinase-1 / metabolism
  • Rats
  • Starvation
  • Stimulation, Chemical

Substances

  • Fructosediphosphates
  • Hexosediphosphates
  • Lactates
  • Lactic Acid
  • fructose 2,6-diphosphate
  • Phosphofructokinase-1
  • Glucose