Monoclonal anti-(T,G)-A--L antibodies: characterization of fine specificity and idiotype expression

Mol Immunol. 1982 Dec;19(12):1551-9. doi: 10.1016/0161-5890(82)90266-8.

Abstract

The technique of polyethylene glycol mediated cell fusion was used to establish 22 monoclonal cell lines secreting anti-(T,G)-A--L antibody. Cell lines were derived from C3H.SW and B10 mice and produced antibody with kappa light chains and predominantly gamma 1, heavy chains. Fine-specificity analysis demonstrated that 15 cell lines made antibodies that also recognize a determinant present on GAT, GT (9:1) and GT (1:1), whereas little, if any, serum antibody demonstrates this cross-reaction. Fourteen antibodies, derived from both B10 and C3H.SW mice, bear idiotypic determinants defined by Lewis anti-[B10 anti-(T,G)-A--L], but only two, both from C3H.SW mice, react with Lewis anti-[C3H.SW anti-(T,G)-A--L]. Adsorption studies indicate that no hybridoma tested bore the complete set of idiotypic determinants defined by either serum.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Antibody Specificity*
  • Cell Line
  • Hybridomas / immunology
  • Immunoglobulin Idiotypes / immunology*
  • Immunosorbent Techniques
  • Mice
  • Mice, Inbred Strains
  • Peptides / immunology*
  • T-Lymphocytes, Helper-Inducer / immunology*

Substances

  • Antibodies, Monoclonal
  • Immunoglobulin Idiotypes
  • Peptides
  • (T,G)-A-L