Novikoff ascites tumor cells contain N-acetyllactosaminide beta 1 leads to 3 and beta 1 leads to 6 N-acetylglucosaminyltransferase activity

J Biol Chem. 1983 Mar 25;258(6):3435-7.


Novikoff ascites tumor cell homogenate was found to catalyze the transfer of [14C]N-acetylglucosamine from UDP-[14C]GlcNAc to asialo-alpha 1-acid glycoprotein. Mucins appeared to be poor acceptors. Methylation and hydrolysis of the product formed in an incubation with UDP-GlcNAc and asialo-alpha 1-acid [3H]glycoprotein yielded 2,4,6-trimethyl [3H]galactose and 2,3,4-trimethyl [3H]galactose, indicating that N-acetylglucosaminyl residues were introduced to position C-3 and C-6 of the terminal galactoses on the glycoprotein. It is concluded that Novikoff cells contain two N-acetylglucosaminyltransferases which might be involved in the synthesis of linear and branched forms of cell surface polylactosaminoglycans and blood group I/i antigenic structures.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cations, Divalent
  • Kinetics
  • Liver Neoplasms, Experimental / enzymology*
  • N-Acetylglucosaminyltransferases / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Substrate Specificity


  • Cations, Divalent
  • N-Acetylglucosaminyltransferases
  • N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
  • N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase