A study of the heat-shock response in Neurospora crassa

Int J Biochem. 1983;15(5):639-49. doi: 10.1016/0020-711x(83)90188-x.

Abstract

1. Neurospora crassa was grown at 28 degrees C for 12 hr and transferred to higher temperatures for 2 hr. 2. Cultures labelled with [35S]methionine showed the synthesis of several new proteins in response to heat-shock at 46 to 48 degrees C. 3. Major polypeptides of approximate Mr 105,000, 99,000, 78,000, 43,000 and 23,000 were detectable in one-dimensional SDS-polyacrylamide slab gel electropherograms. 4. 2-D analysis using isoelectric-focussing in the first dimension and electrophoresis in SDS-polyacrylamide gels in the second led to the resolution of some of the heat-induced polypeptide into multiple spots differing in pI values. 5. mRNA from heat-shocked cells was translated poorly in Wheat Germ extract and rabbit reticulocyte lysate in vitro translation systems.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Physiological*
  • Fungal Proteins / biosynthesis
  • Hot Temperature*
  • Neurospora / physiology*
  • Neurospora crassa / physiology*
  • Peptides / isolation & purification
  • Protein Biosynthesis
  • RNA, Fungal / metabolism
  • RNA, Messenger / metabolism

Substances

  • Fungal Proteins
  • Peptides
  • RNA, Fungal
  • RNA, Messenger