Biogenesis of mitochondria: the mitochondrial gene (aap1) coding for mitochondrial ATPase subunit 8 in Saccharomyces cerevisiae

Nucleic Acids Res. 1983 Jul 11;11(13):4435-51. doi: 10.1093/nar/11.13.4435.


A mitochondrial gene (denoted aap1) in Saccharomyces cerevisiae has been characterized by nucleotide sequence analysis of a region of mtDNA between the oxi3 and oli2 genes. The reading frame of the aap1 gene specifies a hydrophobic polypeptide containing 48 amino acids. The functional nature of this reading frame was established by sequence analysis of a series of mit- mutants and revertants. Evidence is presented that the aap1 gene codes for a mitochondrially synthesized polypeptide associated with the mitochondrial ATPase complex. This polypeptide (denoted subunit 8) is a proteolipid whose size has been previously assumed to be 10 kilodaltons based on its mobility on SDS-polyacrylamide gels, but the sequence of the aap1 gene predicts a molecular weight of 5,815 for this protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / genetics*
  • Amino Acid Sequence
  • Base Sequence
  • DNA, Mitochondrial / genetics*
  • Genes*
  • Mitochondria / enzymology*
  • Molecular Weight
  • Mutation
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics


  • DNA, Mitochondrial
  • Adenosine Triphosphatases

Associated data

  • GENBANK/K00035