Bunyavirus nucleoprotein, N, and a non-structural protein, NSS, are coded by overlapping reading frames in the S RNA

J Gen Virol. 1983 Aug;64 (Pt 8):1705-14. doi: 10.1099/0022-1317-64-8-1705.

Abstract

It has been shown previously, by sequence analysis of the S RNA segment of snowshoe hare (SSH) bunyavirus, that two overlapping open reading frames in the viral complementary sequence code for proteins with molecular weights of 26.8 X 10(3) and 10.5 X 10(3) respectively. In addition to the viral nucleocapsid (N) protein, which is coded by the S RNA, analyses of parental and reassortant bunyavirus-infected cell extracts have shown that the viral S RNA and M RNA species each code for non-structural proteins (NSS and NSM, respectively). In the present report, in vitro translation analyses of the S mRNA species recovered from virus-infected cells indicate that a single size class of mRNA directs the synthesis of N and NSS. Compositional analyses of selected tryptic peptides of N and NSS have provided proof that N is the product of the first open reading frame, and NSS the product of the second.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bunyaviridae / genetics*
  • Capsid / analysis
  • Capsid / genetics
  • Encephalitis Virus, California / genetics*
  • Genes, Viral*
  • Peptides / analysis
  • Protein Biosynthesis*
  • RNA, Messenger / genetics
  • RNA, Viral / genetics*
  • Viral Nonstructural Proteins
  • Viral Proteins / analysis
  • Viral Proteins / genetics*

Substances

  • Peptides
  • RNA, Messenger
  • RNA, Viral
  • Viral Nonstructural Proteins
  • Viral Proteins