Characterization of a phenotypically distinct subpopulation of Leu-2+ cells that suppresses T cell proliferative responses

J Immunol. 1983 Dec;131(6):2757-61.

Abstract

Two new monoclonal antibodies (termed 2D2 and D12) have been used to identify and to analyze phenotypically distinct subpopulations of human T cells. The 2D2 antibody recognized an antigenic determinant closely related, if not identical, to that reactive with the anti-Leu-2 monoclonal antibody. The D12 antibody reacted with a variety of cell types, which included a subpopulation of Leu-2+ (2D2+) T cells. These antibodies were used to isolate four phenotypically distinct T cell populations by sequential cell sorter techniques. Functional analyses demonstrated that the 2D2+D12+ subset was unique in its ability to suppress the antigen-induced proliferation of T cells. These cells also suppressed the proliferative responses of other T cell subsets stimulated with mitogens. Pretreatment of 2D2+D12+ T cells with mitomycin C before culture abrogated the suppressor cell activity of these cells. We propose that the cells within the Leu-2+ cytotoxic/suppressor T cell subpopulation that suppress T cell proliferation are phenotypically distinct and express the 2D2+D12+ membrane antigenic phenotype.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antigen-Antibody Reactions
  • Antigens, Surface / analysis
  • Cell Separation
  • Humans
  • Lymphocyte Activation*
  • Mice
  • Mice, Inbred BALB C
  • Mitogens / pharmacology
  • Phenotype
  • T-Lymphocytes / classification
  • T-Lymphocytes / immunology*
  • T-Lymphocytes, Regulatory / classification
  • T-Lymphocytes, Regulatory / immunology*

Substances

  • Antibodies, Monoclonal
  • Antigens, Surface
  • Mitogens