Echinocandin inhibition of 1,3-beta-D-glucan synthase from Candida albicans

FEBS Lett. 1984 Jul 23;173(1):134-8. doi: 10.1016/0014-5793(84)81032-7.

Abstract

The cyclic peptide antibiotic echinocandin was found to inhibit 1,3-beta-D-glucan synthase activity present in a mixed membrane fraction from Candida albicans. Addition of antibiotic reduced the Vmax of the enzyme, but the Km was unaffected. GTP stimulated enzyme activity approximately 4-fold, but did not affect the percentage inhibition of the enzyme by echinocandin. Treatment of the reaction products with alpha-amylase and beta-glucanase confirmed that the polymer synthesised was 1,3-beta-D-glucan, not glycogen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents*
  • Candida albicans / enzymology*
  • Echinocandins
  • Fungal Proteins*
  • Glucosyltransferases / antagonists & inhibitors*
  • Guanosine Triphosphate / pharmacology
  • Kinetics
  • Membrane Proteins*
  • Peptides / pharmacology
  • Peptides, Cyclic*
  • Schizosaccharomyces pombe Proteins*

Substances

  • Anti-Bacterial Agents
  • Echinocandins
  • Fungal Proteins
  • Membrane Proteins
  • Peptides
  • Peptides, Cyclic
  • Schizosaccharomyces pombe Proteins
  • Guanosine Triphosphate
  • echinocandin B
  • Glucosyltransferases
  • 1,3-beta-glucan synthase