Abstract
The inhibitory effect of inorganic phosphate on the activity determination of isoenzymes of alkaline phosphatase (AP) in diethanolamine (DEA), glycine and 2-amino-2-methyl-1,3-propandiol (AMPD) buffer was studied. This effect depends on the buffer used and isoenzyme investigated. Especially the placental isoenzyme is inhibited; the inhibitory effect in DEA buffer is stronger than in the other buffers used. The requirement of purity for 4-nitrophenylphosphate with respect to its content of inorganic phosphate and conclusions for using control sera enriched with AP isoenzymes are discussed.
MeSH terms
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4-Nitrophenylphosphatase / analysis
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Alkaline Phosphatase / analysis*
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Alkaline Phosphatase / antagonists & inhibitors
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Bone and Bones / enzymology
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Buffers
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Ethanolamines / standards
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Female
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Glycine / standards
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Humans
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Intestine, Small / enzymology
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Isoenzymes / analysis*
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Isoenzymes / antagonists & inhibitors
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Liver / enzymology
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Nitrophenols / standards
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Organophosphorus Compounds / standards
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Phosphates / pharmacology*
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Placenta / enzymology
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Pregnancy
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Propylene Glycols / standards
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Quality Control
Substances
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Buffers
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Ethanolamines
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Isoenzymes
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Nitrophenols
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Organophosphorus Compounds
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Phosphates
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Propylene Glycols
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nitrophenylphosphate
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diethanolamine
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2-amino-2-methyl-1,3-propandiol
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Alkaline Phosphatase
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4-Nitrophenylphosphatase
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Glycine