ADP-ribosylation of nuclear proteins in normal lymphocytes and in low-grade malignant non-Hodgkin lymphoma cells

Eur J Biochem. 1980 Feb;104(1):279-87. doi: 10.1111/j.1432-1033.1980.tb04426.x.


Normal lymphocytes and lymphocytes from patients with low-grade malignant non-Hodgkin lymphoma were isolated from blood by a Percoll gradient procedure. Absence of cell proliferation in both cell types was indicated by very low [3H]thymidine incorporation rates. Determination of endogenous protein-bound single ADP-ribose residues by a radioimmunoassay revealed that the leukemic cells had 2.5-times lower levels of the NH2OH-sensitive and a 4-fold lower amount of NH2OH-resistant ADP-ribose . protein conjugate subfractions, respectively, than normal lymphocytes. By contrast, "total" ADP-ribose transferase activity, as measured in homogenates or permeabilized cells in the presence of DNase, was two-times higher in leukemic cells, whereas activity determined in permeabilized cells in the absence of added DNase was practically identical in both cell types. The apparent discrepancy between ADP-ribose transferase activity and endogenous levels of protein-bound single ADP-ribose residues may be explained in part by an enzyme inhibitor present in normal human lymphocytes. NAD + NADH levels were decreased 2.5-fold in the leukemic cells. This decrease, however, does not explain the reduced levels of mono(ADP-ribose) . protein conjugates since the ratio of protein-bound single ADP-ribose residues to NAD is distinctly different in leukemic lymphocytes compared to normal lymphocytes.

Publication types

  • Comparative Study

MeSH terms

  • ADP Ribose Transferases
  • Adenosine Diphosphate Ribose / metabolism*
  • B-Lymphocytes / metabolism
  • DNA Replication
  • Humans
  • Kinetics
  • Leukemia, Lymphoid / metabolism*
  • Lymphocytes / metabolism*
  • Lymphoma / metabolism*
  • NAD / metabolism
  • Nucleoproteins / metabolism*
  • Nucleoside Diphosphate Sugars / metabolism*
  • Nucleotidyltransferases / metabolism
  • Poly(ADP-ribose) Polymerases
  • Reference Values
  • Thymidine / metabolism


  • Nucleoproteins
  • Nucleoside Diphosphate Sugars
  • NAD
  • Adenosine Diphosphate Ribose
  • ADP Ribose Transferases
  • Poly(ADP-ribose) Polymerases
  • Nucleotidyltransferases
  • Thymidine