Isolation of the chicken thymidine kinase gene by plasmid rescue

Nature. 1980 May 22;285(5762):207-10. doi: 10.1038/285207a0.

Abstract

We have used the bacterial plasmid pBR322 as a vehicle to isolate genes coding for selectable markers from higher eukaryotes. In this way, we have obtained the chicken thymidine kinase (tk) gene as a 2.2-kilobase EcoRI/HindIII insert in BR322. The cloned gene transforms tk- animal cells with an efficiency equal to that of the cloned herpes simplex virus-1 tk gene.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Chickens / genetics
  • Cloning, Molecular / methods*
  • DNA Restriction Enzymes
  • DNA, Recombinant
  • Escherichia coli / genetics
  • Genes*
  • L Cells
  • Mice
  • Plasmids
  • Thymidine Kinase / genetics*
  • Transformation, Genetic

Substances

  • DNA, Recombinant
  • Thymidine Kinase
  • DNA Restriction Enzymes