Insertion of a new gene of viral origin into bone marrow cells of mice

Science. 1980 May 30;208(4447):1033-5. doi: 10.1126/science.6246577.


DNA containing the herpes simplex virus thymidine kinase (HSVtk) gene was used to transform wild-type tk+ mouse L cells to a tk++ status in vitro using methotrexate as a selective agent. HSVtk DNA was also used to transform mouse bone marrow cells in vitro. Transformed marrow cells injected into irradiated and methotrexate-treated recipient mice gave rise to proliferating cells which in some cases dominated the marrow population and which contained HSVtk gene sequences.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bone Marrow / enzymology*
  • Bone Marrow Transplantation
  • DNA, Viral / analysis
  • Drug Resistance
  • Genes, Viral*
  • L Cells
  • Methotrexate / pharmacology
  • Mice
  • Simplexvirus / enzymology
  • Simplexvirus / genetics*
  • Species Specificity
  • Thymidine Kinase / genetics*
  • Transformation, Genetic*


  • DNA, Viral
  • Thymidine Kinase
  • Methotrexate