Construction and Properties of a New Cloning Vehicle, Allowing Direct Screening for Recombinant Plasmids

Mol Gen Genet. 1980;178(2):475-7. doi: 10.1007/BF00270503.

Abstract

pUR2, a certified B2(EK2) vector, allows easy isolation of variants containing cloned EcoRI-fragments. Bacteria harboring plasmids without inserts make blue colonies on indicator-plates, whilst those harboring recombinant plasmids make white colonies.

MeSH terms

  • Cloning, Molecular
  • Escherichia coli / genetics*
  • Genetic Vectors*
  • Plasmids*
  • Recombination, Genetic*