Use of Canonical Variates Analysis in Differentiation of Bacteria by Pyrolysis Gas-Liquid Chromatography

J Gen Microbiol. 1978 Jan;104(1):67-74. doi: 10.1099/00221287-104-1-67.


Low-resolution pyrolysis gas-liquid chromatography (p.g.l.c.) can differentiate genera of aerobic food spoilage bacteria. Multivariate statistical techniques were applied but neither principal components nor furthest neighbour cluster analysis produced a consistent differentiation although both confirmed the reproducibility of p.g.l.c. When the distance between genera was redefined in terms of Mahalanobis D2- a generalized concept taking into account scatter around the mean-good differentiation was observed and could be displayed graphically by plotting the genus group means relative to the first two canonical variate axes. The coefficients of the canonical variates provide a strategy for discriminating between the genus groups. Some practical problems in the identification of unknowns using this technique are discussed.

MeSH terms

  • Bacteria / classification*
  • Chromatography, Gas
  • Food Microbiology
  • Hot Temperature
  • Methods
  • Statistics as Topic