Steady-state kinetics of mouse DNA polymerase alpha

J Biochem. 1980 Jul;88(1):35-8.


Steady-state kinetic studies of DNA polymerase alpha purified from mouse myeloma MOPC104E cells have been carried out. The results of initial velocity analysis with or without sodium pyrophosphate, a product inhibitor, indicated that the reaction mechanism of this enzyme can be categorized as an ordered Bi Bi type where the concentration of the ternary complex is very low.

MeSH terms

  • Animals
  • Cell Line
  • DNA Polymerase II / metabolism*
  • DNA-Directed DNA Polymerase / metabolism*
  • Diphosphates / pharmacology
  • Kinetics
  • Mice
  • Plasmacytoma


  • Diphosphates
  • DNA Polymerase II
  • DNA-Directed DNA Polymerase