Corticosteroid-induced differential regulation of beta-adrenergic receptors in circulating human polymorphonuclear leukocytes and mononuclear leukocytes

J Clin Endocrinol Metab. 1980 Sep;51(3):599-605. doi: 10.1210/jcem-51-3-599.

Abstract

A method of reproducibility measuring human leukocyte beta-adrenergic receptor density and affinity has been developed and applied to the study of receptor regulation in man. The method has the advantages of using a membrane preparation which binds highly specifically and employing techniques such as using low concentrations of [3H]dihydroalprenol, analyzing the data by computer modelling techniques, and providing data from both granulocytes and lymphocytes in the same individual to minimize measurement errors. Using this methodology, human beta-adrenergic receptor regulation is examined. Cortisone acetate was found to induce an acute rise in granulocyte beta-adrenergic receptor density and adenylate cyclase activity and an acute fall in lymphocyte beta-adrenergic receptor density. This potentially differential regulation of a single receptor subtype in two lines of leukocytes has important implications for the study of receptor regulation in man using leukocyte models.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenylyl Cyclases / metabolism
  • Adolescent
  • Adult
  • Cortisone / analogs & derivatives*
  • Dihydroalprenolol / metabolism
  • Female
  • Humans
  • Male
  • Monocytes / metabolism*
  • Neutrophils / metabolism*
  • Receptors, Adrenergic / metabolism*
  • Receptors, Adrenergic, beta / metabolism*

Substances

  • Receptors, Adrenergic
  • Receptors, Adrenergic, beta
  • Dihydroalprenolol
  • Adenylyl Cyclases
  • Cortisone