Resolution, reconstitution and kinetics of the primary action of a hormone receptor

Nature. 1980 Sep 25;287(5780):297-300. doi: 10.1038/287297a0.

Abstract

The beta-adrenergic receptor (R) and the GTP binding protein (G) with which the receptor interacts were obtained in separate soluble fractions. Their recombination produced a hormone responsive system. In the presence of hormone the encounter of R with G in the reconstituted membrane occurs much faster than the subsequent R-induced activation of G.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenylyl Cyclases / metabolism*
  • Animals
  • Carrier Proteins / metabolism*
  • Enzyme Activation
  • Erythrocyte Membrane / metabolism
  • GTP-Binding Proteins
  • Guanosine Triphosphate / metabolism*
  • Guanylyl Imidodiphosphate / metabolism
  • Kinetics
  • Membrane Proteins / metabolism*
  • Protein Binding
  • Receptors, Adrenergic / metabolism*
  • Receptors, Adrenergic, beta / metabolism*
  • Solubility
  • Turkeys

Substances

  • Carrier Proteins
  • Membrane Proteins
  • Receptors, Adrenergic
  • Receptors, Adrenergic, beta
  • Guanylyl Imidodiphosphate
  • Guanosine Triphosphate
  • GTP-Binding Proteins
  • Adenylyl Cyclases