The stimulation of the initiation of DNA synthesis by fibroblast growth factor in Swiss 3T3 cells: interactions with hormones during the pre-replicative phase

J Cell Physiol. 1980 Apr;103(1):77-85. doi: 10.1002/jcp.1041030112.

Abstract

Fibroblast Growth Factor (FGF) stimulates quiescent Swiss 3T3 cells to initiate DNA synthesis and divide. Cells begin to enter the S-phase after a lag of 13--15 hr, and the rate of initiation of DNA synthesis in the population can be quantified by a first order rate constant, k. A subsaturating concentration of FGF may establish the lag phase, while the value of k is dependent on the FGF concentration present during the second half of the lag phase. Insulin and hydrocortisone enhance the effect of FGF by increasing k without changing the lag phase, and they can act when added at any time after FGF. Prostaglandin E1 (PGE1) causes a decrease in k and a lengthening of the lag phase, and acts only when added during the first 8 hr. None of these agents stimulate DNA synthesis in the absence of FGF.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Cyclic AMP / analysis
  • DNA / biosynthesis*
  • Dose-Response Relationship, Drug
  • Drug Synergism
  • Fibroblasts / analysis
  • Fibroblasts / metabolism*
  • Growth Substances / pharmacology*
  • Hydrocortisone / pharmacology
  • Insulin / pharmacology
  • Mice
  • Prostaglandins E / pharmacology
  • Prostaglandins F / pharmacology

Substances

  • Growth Substances
  • Insulin
  • Prostaglandins E
  • Prostaglandins F
  • DNA
  • Cyclic AMP
  • Hydrocortisone