Guanine nucleotide-binding and autophosphorylating activities associated with the p21src protein of Harvey murine sarcoma virus

Nature. 1980 Oct 23;287(5784):686-91. doi: 10.1038/287686a0.


The purified p21src protein of Harvey sarcoma virus shows a guanine nucleotide-binding activity and, in addition, at elevated temperature an autophosphorylating activity at a threonine residue using as phosphoryl donor GTP or dGTP but not ATP or dATP. These biochemical activities are unique among those associated with transforming proteins of RNA-containing or DNA-containing tumour viruses.

MeSH terms

  • Animals
  • Cell Transformation, Viral*
  • Cells, Cultured
  • Guanine Nucleotides / metabolism*
  • Mice
  • Oncogene Protein p21(ras)
  • Phosphoproteins / metabolism
  • Phosphorylation
  • Protein Kinases / metabolism*
  • Sarcoma Viruses, Murine / metabolism*
  • Sarcoma, Experimental / metabolism
  • Substrate Specificity
  • Threonine / metabolism
  • Viral Proteins / metabolism*


  • Guanine Nucleotides
  • Phosphoproteins
  • Viral Proteins
  • Threonine
  • Protein Kinases
  • Oncogene Protein p21(ras)