Identification of a cellular protein substrate phosphorylated by the avian sarcoma virus-transforming gene product

Cell. 1980 Oct;21(3):829-36. doi: 10.1016/0092-8674(80)90446-8.

Abstract

The avian sarcoma virus-transforming gene product (pp60src) appears potentially able to mediate cell transformation via phosphorylation since it is tightly associated with a protein kinase activity. We have searched for and have been able to identify a normal cellular protein that appears to be a substrate of pp60src. The phosphorylation of this protein (34K) in transformation-specific in ASV-transformed cells of both avian and mammalian origin. Moreover, the 34K polypeptide serves as a substrate for the pp60src phosphotransferase activity in vitro and is phosphorylated at a site identical to the major site of phosphorylation in vivo. These data suggest that upon transformation the 34,000-dalton protein is phosphorylated directly as a result of pp60src activity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Avian Sarcoma Viruses / metabolism*
  • Cell Line
  • Cell Transformation, Neoplastic*
  • Cell Transformation, Viral*
  • Chick Embryo
  • Phosphoproteins / analysis
  • Phosphoproteins / metabolism*
  • Phosphorylation
  • Tyrosine / metabolism
  • Viral Proteins / analysis
  • Viral Proteins / metabolism*

Substances

  • Phosphoproteins
  • Viral Proteins
  • Tyrosine