Detection of IgM Antibodies to Cytomegalovirus (CMV) Using an Enzyme-Labelled Antigen (ELA)

J Gen Virol. 1980 Sep;50(1):59-68. doi: 10.1099/0022-1317-50-1-59.

Abstract

We have applied a peroxidase enzyme-labelled antigen (ELA) for the detection of IgM antibodies to cytomegalovirus: microtitre plates were coated with anti-IgM immunoglobulin. The IgM fraction of human serum was selectively bound to the precoated plates and the virus-specific IgM antibody was then detected by the enzyme-labelled antigen. A very efficient technique for the labelling of virus antigen is described. The IgM antibody was detected simply and specifically, Rheumatoid factor IgM did not interfere with this test.

MeSH terms

  • Antibodies, Viral / analysis*
  • Cytomegalovirus / immunology*
  • Cytomegalovirus Infections / immunology
  • Humans
  • Immunoenzyme Techniques
  • Immunoglobulin M / analysis*
  • Rheumatoid Factor / immunology

Substances

  • Antibodies, Viral
  • Immunoglobulin M
  • Rheumatoid Factor