Bacillus subtilis-phage phi 1 overcomes host-controlled restriction by producing BamNx inhibitor protein

Mol Gen Genet. 1980;179(3):463-8. doi: 10.1007/BF00271735.

Abstract

Bacillus amyloliquefaciens N produces two restriction enzymes, BamNI and BamNx. Subtilis-phage phi 1 is strongly restricted by BamNx. We isolated phi 1 rH, a mutant of phage phi 1, which overcame the BamNx-restriction by producing inhibitor. This inhibitor inactivated BamNx specifically and reversibly. The inhibitor directly interacted with BamNx and the inactivation might be the result of formation of a binary complex. The inhibitory activity was sensitive to treatment with trypsin. The molecular weight of the inhibitor protein was estimated to be approximately 20,000 daltons by gel filtration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus subtilis / enzymology
  • Bacteriophages / genetics*
  • DNA Restriction Enzymes / antagonists & inhibitors*
  • Molecular Weight
  • Mutation*
  • Viral Proteins / analysis
  • Viral Proteins / genetics*

Substances

  • Viral Proteins
  • DNA Restriction Enzymes