2-amino-4-phosphonobutyric acid: a new pharmacological tool for retina research

Science. 1981 Jan 9;211(4478):182-5. doi: 10.1126/science.6255566.


Information processing in the vertebrate retina occurs in two separate channels known as ON and OFF channels. When intracellular electrophysiological recordings were obtained from the perfused retina-eyecup preparation of the mud-puppy (Necturus maculosus), the addition of 2-amino-4-phosphonobutyric acid to the bathing medium blocked all responses in the ON channel but left intact the OFF responses including OFF ganglion cell discharge. 2-Amino-4-phosphonobutyric acid blocks the light response of the ON bipolar cell by mimicking the endogenous photoreceptor transmitter.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Action Potentials / drug effects
  • Aminobutyrates / pharmacology*
  • Animals
  • In Vitro Techniques
  • Light
  • Necturus
  • Organophosphorus Compounds / pharmacology*
  • Receptors, Neurotransmitter / drug effects
  • Retina / cytology
  • Retina / drug effects*
  • Retina / physiology
  • Retina / radiation effects
  • Vision, Ocular / drug effects


  • Aminobutyrates
  • Organophosphorus Compounds
  • Receptors, Neurotransmitter
  • 2-amino-4-phosphonobutyric acid