Formulation and some biological uses of a buffer mixture whose buffering capacity is relatively independent of pH in the range pH 4-9

J Biochem Biophys Methods. 1980 Sep;3(3):143-50. doi: 10.1016/0165-022x(80)90013-5.


A mixture is described which has a buffering capacity which is essentially independent of pH in the range pH 4.0-9.0. It is shown how this buffer mixture may be used to determine the force-flux relationship of proton transfer between two aqueous phases separated by a phospholipid bilayer in vesicular systems and so demonstrate that this relationship is linear over a wide range of delta mu approximately H+. The buffer mixture can, furthermore, be employed to determine the volume enclosed within a vesicular preparation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Buffers*
  • Hydrogen-Ion Concentration
  • Liposomes
  • Methods
  • Protons


  • Buffers
  • Liposomes
  • Protons