Chromatin structure of the histone genes of D. melanogaster

Cell. 1981 Feb;23(2):401-9. doi: 10.1016/0092-8674(81)90135-5.

Abstract

We have examined the chromatin structure of the histone gene repeat of D. melanogaster using an indirect end-labeling technique. Our results show that each DNA segment of the repeat is packaged into a precisely defined and characteristic structure, as follows: the nontranscribed spacers display a "normal" chromatin arrangement, with each nucleosome precisely positioned on the underlying DNA sequence; the 5' ends of all five histone genes are in an exposed configuration, highly sensitive to both micrococcal nuclease and DNAase I; and the genes have an "altered" chromatin structure, as indicated by the weak and irregularly spaced nuclease cuts. This well-defined chromatin arrangement is established early in development and is stably maintained throughout the remainder of the D. melanogaster life cycle.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bacterial Proteins*
  • Chemical Phenomena
  • Chemistry
  • Chromatin / analysis*
  • DNA Restriction Enzymes
  • Deoxyribonuclease BamHI
  • Deoxyribonuclease I
  • Deoxyribonucleases
  • Deoxyribonucleases, Type II Site-Specific*
  • Drosophila melanogaster
  • Embryo, Nonmammalian / analysis
  • Endonucleases
  • Genes*
  • Histones / genetics*
  • Micrococcal Nuclease
  • Nucleosomes / analysis
  • Transcription, Genetic

Substances

  • Bacterial Proteins
  • Chromatin
  • Histones
  • Nucleosomes
  • Deoxyribonucleases
  • Endonucleases
  • DNA Restriction Enzymes
  • Deoxyribonuclease BamHI
  • Deoxyribonuclease I
  • BglII endonuclease
  • Deoxyribonucleases, Type II Site-Specific
  • Micrococcal Nuclease