Hormone effects on transport in cultured epithelia with high electrical resistance

Am J Physiol. 1981 Mar;240(3):C103-5. doi: 10.1152/ajpcell.1981.240.3.C103.

Abstract

Three continuous lines of amphibian epithelial cells form epithelia with a high transepithelial resistance (greater than 4,000 omega . cm2) in culture. The cell lines are TB-M and TB-6c, derived from the urinary bladder of Bufo marinus, and A6, derived from the kidney of Xenopus laevis. Short-circuit current is equivalent to net mucosa-to-serosa sodium transport in two cell lines and slightly exceeds sodium transport in epithelia formed by TB-6c cells. None of the cell lines has an adenylate cyclase response or a transport or permeability response to vasopressin. Water permeability is low in all three cell lines and is not affected by adenosine 3',5'-cyclic monophosphate (cAMP). In the three lines of cells, cAMP and aldosterone each increases short-circuit current with a time course similar to that seen in naturally occurring epithelia. In contrast to the toad urinary bladder and epithelia of line TB-M in which the aldosterone stimulation of short-circuit current is associated with a fall in transepithelial resistance, there is no change in resistance across epithelia of lines TB-6c and A6. There is also a striking difference in the sensitivity of the three lines to inhibition of short-circuit current by amiloride.

MeSH terms

  • Aldosterone / pharmacology
  • Amiloride / pharmacology
  • Animals
  • Biological Transport, Active / drug effects
  • Bufo marinus
  • Cell Line
  • Cyclic AMP / metabolism*
  • Kidney
  • Membrane Potentials / drug effects
  • Sodium / metabolism*
  • Time Factors
  • Urinary Bladder
  • Vasopressins / pharmacology
  • Xenopus laevis

Substances

  • Vasopressins
  • Aldosterone
  • Amiloride
  • Sodium
  • Cyclic AMP